tk gene中文是什么意思
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用"tk gene"造句"tk gene"怎么读"tk gene" in a sentence
中文翻译手机版
- 胸苷激酶基因
- "tk"中文翻译 TK =tank.
- "gene"中文翻译 n. 【生物学】基因。 dominant gene显性基 ...
- "tk" 中文翻译 : TK =tank.
- "airhitam tk" 中文翻译 : 亚逸依淡湾
- "back tk" 中文翻译 : 后排队员
- "dj tk" 中文翻译 : 外面的世界 女声版; 我可以抱你吗
- "hs-tk" 中文翻译 : 单纯疱疹-胸腺激酶
- "m tk" 中文翻译 : M Tk = medium tank 中型坦克。
- "tk tokela" 中文翻译 : 托克劳
- "tk (telecine)" 中文翻译 : 电视电影
- "tk cell" 中文翻译 : tk细胞,杀伤性t细胞; 杀伤性t细胞
- "tk glass" 中文翻译 : 特种冕玻璃
- "tk remix" 中文翻译 : 艾晴晴 - 无路可退; 爱啦啦; 被爱伤过的人; 怀念你; 牵挂你的人是我; 情深缘浅-爽版; 容中尔甲-高原红; 我可以抱你; 我想找个女朋友; 我要的世界; 星楼的星座; 杨坤-无所谓; 原来你什么都不想要; 云剑-千里之外; 张栋梁 - 只因为你; 张信哲-别怕我伤心; 只因为你-张栋梁
- "tk rwmix" 中文翻译 : 铃儿响叮铛
- "tk tokelau" 中文翻译 : 托克劳群岛(域名)
- "tk track" 中文翻译 : 音轨
- "tk-208" 中文翻译 : 米的手台
- "tk细胞" 中文翻译 : t killer cell tk cell; tk cell
- "gene" 中文翻译 : n. 【生物学】基因。 dominant gene显性基因。
- "is gene" 中文翻译 : is基因
- "t gene" 中文翻译 : t基因
- "bangladesh taka(tk" 中文翻译 : 孟加拉国塔卡
- "blood group antibody tk" 中文翻译 : tk血型抗体
- "blood group antigen tk" 中文翻译 : tk血型抗原
- "dj rainman tk remix" 中文翻译 : 老公我爱你
例句与用法
- Tk gene mutation test
Tk基因突变试验 - The tk gene contained an open reading frame of 957 bp encoding a 318 aa protein . upstream of the tk orf , three putative gc boxes are located at positions - 22 , - 166 and - 199 . a potential poly a signal begins 110 nucleotides downstream from the termination codon at position 1306
在tkorf上游- 22 、 - 166 、 - 199位存在3个gc框样序列,在终止密码子下游第110个核苷酸处的1306位存在有多聚腺苷加尾信号。 - Three pairs of primers were designed according to the sequences pulished by the genebank in order to amplifiy gd , ge and tk gene of the pseudorabies virus min - a strain . the gd , ge and tk gene were obtained by polymerase chain reaction ( pcr ) , and then cloned into the pgem - t easy vector
参考genebank收录的伪狂犬病病毒gd 、 ge 、 tk基因的序列设计了三对引物,对prvmin - a株进行了pcr扩增,扩增产物克隆于pgem - teasy载体。 - In this study , a 1 . 7kb kpni fragment and a lacz gene expression cassette carrying the e . coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ) . the new transfer vector was called puni - lacz . the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure
本研究以呈ge ~ -表型的经典弱毒疫苗bartha - k61株为亲本株,在通用prv转移载体pbdtk - uni的基础上,在其多克隆位点中插入由sv40早期启动子控制下的lacz基因表达盒,同时将下游同源臂增加了一个1 . 7kb的kpni片段,使上下游同源臂的长度都超过了1kb ,构建了一个新的转移载体puni - lacz 。 - Was multiplied and the tk gene was cloned . the cloned tk gene was retrieved by proper restrictive hemodynamics . the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin . then , the specificity and sensitivity of tk gene probe were detected with dot blot hybridization . the sequence of tk gene of nm98a strain was analysed . the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99 . 7 %
本研究中首次对iltv - nm98a株的tk基因进行了克隆和序列分析,结果表明: iltv - nm98a株tk基因的核苷酸序列与已发表的iltvtk基因的核苷酸序列具有高度的同源性,两者之间仅相差4个核苷酸,同源性高达99 . 7 ,从而证实了iltvtk基因是高度保守的,为iltvtk基因核酸探针的制备提供了有力的依据。 - In this study , iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois . a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ) . the product of pcr was linked with suitable plasmid . then , the recombined plasmid was converted to escherichia coli . the converted escherichia coli
根据已发表的iltvtk基因的核苷酸序列设计一对pcr引物,以增殖的两株iltv的dna为模板,分别对它们的tk基因进行pcr扩增。将回收的pcr产物连接到适当的质粒载体上,转化感受态大肠杆菌,通过筛选对iltvtk基因的阳性克隆进行扩增培养。
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